Biochemical analysis methods overview for biotech and artificial intelligence development (part 5)

Let’s continue going over the basic knowledge required for developing one or more ultra-low cost biochemical composition determination technologies—for advancing human immortality biotech, neurotech, and artificial intelligence. Let’s rock!

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Microscopy Analysis entails, Scanning Electron Microscopy, Scanning electron microscopy paired with Energy Dispersive Spectroscopy Microscopy, Surface Analysis Laboratory Techniques, Fibre Analysis, Optical Microscopy Analysis, Optical Profilometry, Transmission Electron Microscopy, Confocal Laser Scanning Microscopy, Energy Dispersive X-Ray Analysis (EDX), Microscopy of Medical Devices, Microscopy of Thermal Processes, Surface and Interface Science, Digital Image Analysis, Confocal Raman Mapping, Cryo-Electron Microscopy (EM) Imaging, and Time Lapse Imaging. I’ll look into all of those.

Scanning electron microscopy (SEM) is used to study surfaces and particles. Interpreting SEM images is not always clear and direct. For examining the internal structure of samples, a variety of cross-sectioning techniques is used in SEM. Scanning electron microscopy is used with other techniques for materials identification, such as Scanning electron microscopy – Energy Dispersive X-ray (SEM/EDX), Remote View Scanning Electron Microscopy, Cryo Scanning Electron Microscopy (Cryo-SEM), Infra-red (FTIR) Microscopy or RAMAN Microscopy, Time-of-Flight Secondary Ion Mass Spectrometry (SIMS). I’ll look into whether and how scanning electron microscopy and its related materials analysis techniques are useful for biomatter composition analysis and determination.

When you have 1mm by 1mm by 1mm living or dead biomatter cube, how do you cheaply and quickly identify all the biochemicals in that biomatter? I’ll answer that question.

Scanning electron microscopy and Energy Dispersive Spectroscopy (SEM/EDS) combined is for microscopy analysis, that provides resolution of 1.0 nm by scanning electron microscope (SEM) and 0.8 nm by a Scanning Transmission Electron Microscope (STEM), which are small enough to see large enough molecules; atoms have an average radius of about 0.1 nm. A Quanta 250 FEG from FEI Company and a Quantax micro-analysis system from Bruker can be the equipment used in a SEM/EDS pairing; I’ll look into those SEM/EDS instruments and companies. Using a scanning transmission electron detector (STEM) allows forming images using electrons passing through a thin sample. WetSTEM system enables analyzing web samples. Environmental Scanning Electron Microscopy (ESEM) is used for imaging wet materials. I’ll look into everything relevant in Scanning electron microscopy and Energy Dispersive Spectroscopy, and assess whether and how useful they can be in cheaply and quickly identifying or determining biochemicals in a piece of biomatter.

For now, my hunch is that since electron microscopes and electron microscopy are so expensive, I doubt they can be used in cheaply, quickly, and completely identifying all the biochemicals in biomatter. On the Internet, it says, electron microscopes cost US$75,000 – US$10,000,000; a new scanning electron microscope (SEM) costs $70,000 to $1,000,000, while a used scanning electron microscope can cost $2,500 to $550,000 depending on condition. US$75,000 is not out of the price range for a corporate biotech laboratory in the U.S., even for a small and low-budget one, but can a US$75,000 brand-new scanning electron microscope be used to identify all the quadrillions of biochemicals in a dead cold-temperature human body in 24 hours or in several days? Probably not. The current estimate on the Internet is that a living human body has 37.2 trillion cells, with 86 billion cells in the human brain; if the human body has 37 trillion cells, it probably has hundreds of a quadrillion extracellular biochemicals at any given time—carbohydrates, proteins, lipids (fats), and nucleic acids. Well, nucleic acids are in the nucleuses or nuclei of cells, so those are not counted in extracellular biochemicals. The biological tissues are made up of cells and extracellular matrices, which are made of biochemicals or biomolecules; there must be a lot of extracellular biomolecules in the human-body tissues, how many exactly?

I’ll continue in part 6.

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Allen Young

The transhumanistic Asian-American man who publicly promotes and advances AI, robotics, human body biotech, and mass-scale outer space tech.